Heterologous Expression

Caracterización funcional de la podocalicina humana expresada en sistemas heterólogos

Fluorescence Microscopy / Cell Biology / Transmission Electron Microscopy / Mammalian Cell Culture / Genetic Engineering / Green Fluorescent Protein / Transfection / Heterologous Expression / Podocalyxin / Podocalicina humana / Green Fluorescent Protein / Transfection / Heterologous Expression / Podocalyxin / Podocalicina humana

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Biochemical Characterization of an L-Xylulose Reductase from Neurospora crassa

Kinetics / Gene expression / Multidisciplinary / Neurospora crassa / Glucose Metabolism / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway

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Heterologous Expression of Trypanosoma cruzi trans-Sialidase in Leishmania major Enhances Virulence

Immune response / Gene expression / Biological Sciences / Infection and immunity / Virulence / Genetic Testing / Mice / Female / Animals / Infection / Glycoproteins / Chagas disease / Enzyme / Neuraminidase / Immune system / Trypanosoma Cruzi / Virulence factor / Cutaneous Leishmaniasis / Sialic Acid / Heterologous Expression / Leishmania Major / Trans-Sialidase / Genetic Testing / Mice / Female / Animals / Infection / Glycoproteins / Chagas disease / Enzyme / Neuraminidase / Immune system / Trypanosoma Cruzi / Virulence factor / Cutaneous Leishmaniasis / Sialic Acid / Heterologous Expression / Leishmania Major / Trans-Sialidase

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Initial characterization of a recombinant kynureninase from< i> Trypanosoma cruzi</i> identified from an EST database

Genetics / Mass Spectrometry / Trypanosomes / Protein Structure and Function / PCR / Humans / Sequence alignment / Circular Dichroism / Animals / Gene / Bp / Chagas disease / Enzyme / EST / Spectrum / Trypanosoma Cruzi / Molecular Mass / Lb / Amino Acid Sequence / NAD / Open Reading Frame / Heterologous Expression / Recombinant Proteins / SDS PAGE / Kyneurase / Glycosyl Hydrolases / Reading Frames / Differential expression / Molecular Sequence Data / Humans / Sequence alignment / Circular Dichroism / Animals / Gene / Bp / Chagas disease / Enzyme / EST / Spectrum / Trypanosoma Cruzi / Molecular Mass / Lb / Amino Acid Sequence / NAD / Open Reading Frame / Heterologous Expression / Recombinant Proteins / SDS PAGE / Kyneurase / Glycosyl Hydrolases / Reading Frames / Differential expression / Molecular Sequence Data

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Transport processes in Plasmodium falciparum-infected erythrocytes: potential as new drug targets

Microbiology / Zoology / Transport Properties / Animals / Host-parasite interactions / Membrane transport proteins / Substrate Specificity / Plasmodium falciparum / For / Veterinary Sciences / Heterologous Expression / Erythrocytes / Drug Targeting / Plasma Membrane / Membrane transport proteins / Substrate Specificity / Plasmodium falciparum / For / Veterinary Sciences / Heterologous Expression / Erythrocytes / Drug Targeting / Plasma Membrane

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Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme

Gene expression / Multidisciplinary / Neurospora crassa / Biological Sciences / Vancomycin / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data

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Initial characterization of a recombinant kynureninase from Trypanosoma cruzi identified from an EST database

Genetics / Mass Spectrometry / Trypanosomes / Protein Structure and Function / PCR / Humans / Sequence alignment / Circular Dichroism / Animals / Gene / Bp / Chagas disease / Enzyme / EST / Spectrum / Trypanosoma Cruzi / Molecular Mass / Lb / Amino Acid Sequence / NAD / Open Reading Frame / Heterologous Expression / Recombinant Proteins / SDS PAGE / Kyneurase / Glycosyl Hydrolases / Reading Frames / Differential expression / Molecular Sequence Data / Humans / Sequence alignment / Circular Dichroism / Animals / Gene / Bp / Chagas disease / Enzyme / EST / Spectrum / Trypanosoma Cruzi / Molecular Mass / Lb / Amino Acid Sequence / NAD / Open Reading Frame / Heterologous Expression / Recombinant Proteins / SDS PAGE / Kyneurase / Glycosyl Hydrolases / Reading Frames / Differential expression / Molecular Sequence Data

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Biochemical Characterization of an L-Xylulose Reductase from Neurospora crassa

Kinetics / Gene expression / Multidisciplinary / Neurospora crassa / Glucose Metabolism / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway

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Initial Characterization of a Recombinant Kynureninase From Trypanosoma Cruzi Identified From An EST Database

Genetics / Mass Spectrometry / Trypanosomes / Protein Structure and Function / PCR / Humans / Sequence alignment / Circular Dichroism / Animals / Gene / Bp / Chagas disease / Enzyme / EST / Spectrum / Trypanosoma Cruzi / Molecular Mass / Lb / Amino Acid Sequence / NAD / Open Reading Frame / Heterologous Expression / Recombinant Proteins / SDS PAGE / Kyneurase / Glycosyl Hydrolases / Reading Frames / Differential expression / Humans / Sequence alignment / Circular Dichroism / Animals / Gene / Bp / Chagas disease / Enzyme / EST / Spectrum / Trypanosoma Cruzi / Molecular Mass / Lb / Amino Acid Sequence / NAD / Open Reading Frame / Heterologous Expression / Recombinant Proteins / SDS PAGE / Kyneurase / Glycosyl Hydrolases / Reading Frames / Differential expression

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LjLHT1.2—a mycorrhiza-inducible plant amino acid transporter from Lotus japonicus

Plant Nutrition / Gene expression / Biological Sciences / Environmental Sciences / Arbuscular mycorrhizal fungi / AM fungi / Nitrogen / Amino Acid Profile / Heterologous Expression / Arbuscular Mycorrhiza Fungi / AM fungi / Nitrogen / Amino Acid Profile / Heterologous Expression / Arbuscular Mycorrhiza Fungi

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Heterologous cross-expression of oxygenase and glycosyltransferase genes in streptomycetes, producing angucyclic antibiotics

Gene expression / Streptomyces / Plasmids / Antibacterial activity / Anti-Bacterial Agents / Glycosyltransferases / Heterologous Expression / Glycosyltransferases / Heterologous Expression

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Recombinant expression, activity screening and functional characterization identifies three novel endo-1,4-β-glucanases that efficiently hydrolyse cellulosic substrates

Applied microbiology / Gene expression / Multidisciplinary / Cellulose / Cellulase / Aspergillus niger / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Heterologous Expression / Recombinant Proteins / Ascomycota / Production Cost / BASIDIOMYCOTA / Aspergillus niger / Enzyme / Applied Microbiology and Biotechnology / Molecular cloning / Heterologous Expression / Recombinant Proteins / Ascomycota / Production Cost / BASIDIOMYCOTA

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Expression of a synthetic gene encoding a Tribolium castaneum carboxylesterase in Pichia pastoris

Escherichia coli / Protein Secretion / Animals / Pichia pastoris / Molecular cloning / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology

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Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes

Genetics / Microbiology / Functional Analysis / Intelligent Transport System / Saccharomyces cerevisiae / Membrane transport proteins / Molecular Characterization / Carboxylic Acids / Heterologous Expression / Lactic Acid / Plasma Membrane / Membrane transport proteins / Molecular Characterization / Carboxylic Acids / Heterologous Expression / Lactic Acid / Plasma Membrane

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Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes

Genetics / Microbiology / Functional Analysis / Intelligent Transport System / Saccharomyces cerevisiae / Membrane transport proteins / Molecular Characterization / Carboxylic Acids / Heterologous Expression / Lactic Acid / Plasma Membrane / Membrane transport proteins / Molecular Characterization / Carboxylic Acids / Heterologous Expression / Lactic Acid / Plasma Membrane

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An N-terminal peptide extension results in efficient expression, but not secretion, of a synthetic horseradish peroxidase gene in transgenic tobacco

Plant Biology / Tobacco / Ecology / Western blotting / Protein Targeting / Peroxidase / Tobacco Use / Cell Fractionation / Enzyme / Nicotiana Tabacum / Isoenzymes / Enzyme activity / Plant Development / Annals / Amino Acid Sequence / Recombinant Protein / Heterologous Expression / Subcellular Fractions / Recombinant Proteins / PLANT PROTEINS / Transgenic plant / Plant Leaves / Peroxidase / Tobacco Use / Cell Fractionation / Enzyme / Nicotiana Tabacum / Isoenzymes / Enzyme activity / Plant Development / Annals / Amino Acid Sequence / Recombinant Protein / Heterologous Expression / Subcellular Fractions / Recombinant Proteins / PLANT PROTEINS / Transgenic plant / Plant Leaves

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